Following heatmap present the correlation between distances computed from multiple sequence alignment (MSA) versus pairwise local alignment for revised 599Nts dataset (MSA is available here and sequence file is here).
Details on MSA based distance computation is at http://salsafungiphy.blogspot.com/2013/06/pairwise-distance-calculation.html
Details on pairwise local alignment based distance computation is at http://salsafungiphy.blogspot.com/2012/10/pairwise-distances-from-multiple.html
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